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. 2019 Oct 22;294(48):18232–18243. doi: 10.1074/jbc.RA119.011109

Figure 5.

Figure 5.

Binding capacities of the cell-surface integrins expressed in HDF cells to fibrillin-1 fragments. For all panels, x axis denotes the concentration of purified fibrillin-1 fragment. A, left panel indicates cell adhesion levels of HDF cells in the absence of treatment to all three fibrillin-1 fragments: WT, disease-causing mutant and RGE knockout. Right panel indicates complete integrin blockade of all relevant integrins using ALULA, PID6, AXUM2, and C8. Solid line denotes adhesion of HDF cells in the absence of antibody treatments; dotted line indicates integrin blockade for cells plated on the specified fragment. B, cell adhesion of integrin αvβ5 in HDF cells, completed in the presence of P1D6, AXUM2, and compound C8. C, cell adhesion of integrin αvβ3 in HDF cells, completed in the presence of ALULA, P1D6, and compound C8. D, cell adhesion of integrin α5β1 in HDF cells, completed in the presence of ALULA, AXUM2, and C8. Negative data for integrin binding to the RGE knockout is not indicated in panels B–D. Data represents mean of 2 biological replicates ± S.E.