Table 1.

Antibody blockade for integrin isolation in cell-adhesion assays

Cells were exposed to combination antibody treatments for isolation of specific integrin-mediated adhesion. All antibodies were added at 10 μg/ml to cells suspended in FBS-free medium and incubated on ice for 10 min before plating on fibrillin-1–coated plates. C8 is a small molecule inhibitor of αvβ1 and was added at 10 μm.

Integrin	Cell line	Antibodies for integrin isolation	Antibodies for complete blockade
α5β1	SW480	ALULA	ALULA + P1D6
αvβ5	SW480	P1D6	ALULA + P1D6
αvβ3	SW480-β3	ALULA + P1D6	ALULA + P1D6 + AXUM2
αvβ6	SW480-β6	ALULA + P1D6	ALULA + P1D6 + 3G9
α8β1	SW480-α8	ALULA + P1D6	ALULA + P1D6 + YZ83
αvβ8	SW480-β8	ALULA + P1D6	ALULA + P1D6 + ADWA-11
αvβ1	HDF	ALULA + P1D6 + AXUM2	L230 + P5D2
α5β1	HDF	L230	L230 + P5D2
αvβ5	HDF	P1D6 + AXUM2 + C8	ALULA + P1D6 + AXUM2 + C8
αvβ3	HDF	ALULA + P1D6 + C8	ALULA + P1D6 + AXUM2 + C8
αIIbβ3	Human platelets	L230 + P5D2	L230 + P5D2 + 10E5