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Oligonucleotide primers used to synthesize genes encoding Tk-hefu derivatives
Restriction sites are underlined, stop codons are in italics, enteropeptidase cleavage site-encoding codons are in bold type, and the differing codons are shown on a gray background. The following pairs of primers were used to amplify the target sequences: Tk-hefu-2, f1 and rev1; Tk-hefu-3, f1 and rev2; and Tk-hefu-4, f2 and rev1.
