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. 2019 Sep 19;294(48):18360–18371. doi: 10.1074/jbc.RA119.010222

Figure 1.

Figure 1.

A conserved residue in the N-terminal region of Bcd1 is important for cell growth. A, domain organization of Bcd1 and ZNHIT6. B, serial dilution growth assay of yeast expressing BCD1 under a doxycycline (Dox) repressible promoter (tetO7::BCD1), and supplemented with the indicated plasmids. C, Western blot analysis of Bcd1 and its variants. Total protein was resolved on a Mini-PROTEAN TGX Stain-Free gel (Bio-Rad) and imaged prior to the transfer to serves as loading control. Anti-HA intensity is normalized to the total protein and reported under each lane. D, serial dilution growth assay of tetO7::BCD1 cells supplemented with the indicated plasmids. E, Western blot analysis of Bcd1 and Bcd1-D72A. The anti-HA levels relative to the loading control is reported under each lane. F, the inflection temperature (Ti) of Bcd1 and Bcd1-D72A as a function of increasing temperature. The peaks in the first derivative view correspond to the Ti of the proteins.