Figure 4.

Bcd1-D72A variant abrogates the production of box C/D snoRNAs. A, Northern blotting analyses of steady-state expression levels of box C/D and box H/ACA snoRNAs in tetO7::BCD1 cells treated with doxycycline and containing an empty vector (lanes 1–3), or a vector expressing Bcd1 (lanes 4–6), or Bcd1-D72A (lanes 7–9). B, quantification of data shown in A as normalized to scR1. Lane 6 was excluded from data analyses. Graph bars represent the mean and S.D. from at least two biological replicates. Significance is relative to the WT and was determined using an unpaired t test. n.s., nonsignificant; p > 0.05; *, p < 0.05; **, p < 0.01.