Figure 5.

Dnmt1 expression is regulated by Myc in SCLL cells. A, organization of the Dnmt1 locus in mice. The positions of the primer pairs within the promoter (P2) and intron 1 (P1), regions used for ChIP, are indicated by the opposing arrows. Compared with the IgG control, there is significant enrichment of Myc binding in the Dnmt1 promoter region (P2) in both BBC2 and ZNF112 cells. B, knockdown of Myc using two different shRNAs shows a concomitant reduction in Dnmt1 expression levels. As a result of Myc knockdown, levels of miR-150-5p show a proportional increase compared with cells expressing a scrambled control (left panel, bottom). Western blot analysis (right panel) shows the relative knockdown levels of Myc using the two shRNAs and the proportional reduction in Dnmt1 and Myb levels.