Figure 7.

The ZIKV-induced IFN level was regulated by ADAR1 and partially contributed to the ADAR1 proviral effect. A, real-time PCR to measure the IFN-β mRNA levels. Control cells and ADAR1 KO cells were mock-infected or infected with ZIKV at MOI 3. Cells were collected at the indicated time points for RNA extraction and qRT-PCR. B, Western blot analysis. IFNAR1^KO and STAT1^KO cells were stimulated by IFN-β. At 24 h, cells were harvested for Western blotting using the indicated antibodies. GAPDH was probed as an internal control. C and D, viral replication levels. Control cells, IFNAR1^KO, and STAT1^KO cells were transfected with siNC or siADAR1. At 48 h post-transfection, cells were infected with ZIKV at MOI 3. At 24 h p.i., the cells were collected for Western blotting using antibodies against ADAR1, p-PKR, PKR, p-eIF2α, eIF2α, E, or GAPDH (C), and the supernatants were collected for the plaque assay (D). Data are shown as means ± S.D. (error bars) of three independent experiments. **, p < 0.01; ***, p < 0.001.