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. 2019 Oct 21;294(48):18168–18180. doi: 10.1074/jbc.RA119.009113

Table 2.

Sequences of oligonucleotides used in cloning

Gene Sequence (5′–3′)
ADAR1-p110 5F: ATTTGCGGCCGCATGGCCGAGATCAAGGAGAAAAT
3R: CGCGGATCCCTATACTGGGCAGAGATAAAAG
ADAR1-p150 5F: ATTTGCGGCCGCATGAATCCGCGGCAGGGGTATT
3R: ATTTGCGGCCGCCTATACTGGGCAGAGATAAAA
ADAR1 (sgRNA2) mutation 5F: GACAATTCTGCTCGAAGAAGCCAAAGCC
3R: GGCTTTGGCTTCTTCGAGCAGAATTGTC
ADAR1 (H910Q/E912A) mutation 5F: CAATGACTGCCAGGCAGCAATAATCTC
3R: GGGAGATTATTGCTGCCTGGCAGTC
ADAR1-p110N 5F: ATTTGCGGCCGCATGGCCGAGATCAAGGAGAAAATC
3R: CGCGGATCCTCACTTATCGTCGTCATCCTTGTAATCACCACCACCGTTCTCCCCAATCAAGACACGGAG
shPKR90 5F: GATCCCCGCAGGGAGTAGTACTTAAATATTCAAGAGATATTTAAGTACTACTCCCTGCTTTTTA
3R: AGCTTAAAAAGCAGGGAGTAGTACTTAAATATCTCTTGAATATTTAAGTACTACTCCCTGCGGG
shPKR405 5F: GATCCCCGCATGGGCCAGAAGGATTTCATTCAAGAGATGAAATCCTTCTGGCCCATGCTTTTTA
3R: AGCTTAAAAAGCATGGGCCAGAAGGATTTCATCTCTTGAATGAAATCCTTCTGGCCCATGCGGG