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. 2019 Oct 11;294(48):18046–18056. doi: 10.1074/jbc.RA119.011008

Figure 5.

Figure 5.

Analysis of the shortened region flanking the internal initiation site. A, schematic representation of the starting full-length (−85, +93) and short (−40, +27) reporter constructs that retained prototypic activity in the eNISTmAb. NNN designates the start codon. B, plate reader analysis of the various start codons' full-length and short constructs from the eNISTmAb. The results and standard deviation (error bars) from four independent experiments are shown. Fluorescence values are normalized to optical density of the cultures and corrected as described under “Experimental procedures.” AU, arbitrary units. C, schematic representation of the starting full-length (−85, +93) and short (−40, +27) reporter constructs in adalimumab. NNN designates the start codon. D, plate reader analysis of the various start codons' full-length and short constructs from adalimumab. The results and standard deviation (error bars) from four independent experiments are shown. Fluorescence values are normalized to optical density of the cultures and corrected as described under “Experimental procedures.” AU, arbitrary units.