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. 2019 Oct 16;294(48):18421–18434. doi: 10.1074/jbc.RA119.011187

Figure 2.

Figure 2.

No changes in swelling-induced activation of TRPV4 upon phosphorylation. A–F, representative I/V curves of TRPV4-mediated activity in control solution or in hyposmotic solution. Control solution is shown in black and hyposmotic (−100 mosm) in red. A–C, activity with PKA-, PKC-, or PKG activators (right). D–F, activity with PKA, PKC, or PKG inhibitors (right). G, current activity, I, at −85 mV obtained after exposure to −100 mosm is shown as bars. Filled red bars, hypotonic solution–induced TRPV4-mediated activity without drug; open bars, response with drug. H, experimental paradigm. Application of a hyposmotic gradient is indicated by a blue bar, and drug application is shown by a pink bar. Arrows indicate when current activity was recorded. N.S., not significant (p > 0.05), one-way ANOVA, n = 9–12 oocytes. Error bars, S.D. (bar graphs) or S.E. (I/V curves).