Figure 8.

PLA₂ activation is required for swelling-induced TRPV4 activation in Müller cells. A, representative raw trace of response recorded in Fura-2AM–loaded retinal ganglion cells. Activation of PLA₂ (sc-3034) is indicated by the pink bar. B, summary for results shown in A. Shown are mean) values before PLA₂ activation (Ctrl) and the change in [Ca²⁺]_i during stimulation (sc-3034), n = 29 cells. C, representative raw trace of response recorded in Müller cells with summary of results shown in D, n = 13 cells. E, representative raw trace of responses recorded in Müller cells verifying their TRPV4 origin. The evoked PLA₂ activation-induced responses are abolished in the presence of HC-06. F, summary of results in E, n = 10 cells. G, representative raw trace of response recorded in Müller cells after pretreatment with a PLA₂ inhibitor (ONO-RS-089, indicated by the pink bar) prior to application of an osmotic gradient (−20 mosm, indicated by the blue bar) and after ONO-RS-089 washout. H, summary of results from G presenting the increase in swelling-induced calcium levels after washout of PLA₂ blocker, n = 14 cells. N.S., not significant (p > 0.05); **, p < 0.01; ***, p < 0.001; two-sample t test. Error bars, S.D.