Figure 4.

AhR recruits PPARγ upon domain–domain interactions. A, mapping of the PPARγ domain responsible for binding AhR. GST-AhR (full length (FL)) and His-PPARγ truncations were expressed in E. coli individually followed by protein purification. Proteins were mixed for Ni²⁺ pulldown followed by Western blotting. Input (5%) was detected using the indicated antibodies. Protein bands of interest are marked with stars. B and C, mapping of the AhR domain responsible to interact with PPARγ. His-PPARγ (full length) and GST-AhR truncations were expressed in E. coli individually followed by protein purification. Proteins were mixed followed by immunoprecipitation with the indicated antibodies and Western blotting. Input (5%) was detected using the indicated antibodies. Protein bands of interest are marked with stars. IP, immunoprecipitation; IB, immunoblotting.