Fig. 4.
NPARC is sensitive to small but reproducible Tm-shifts. The plot compares the effect size measure used by NPARC, namely RSS0 − RSS1 (y axis), to the Tm-difference (x axis) for those proteins in the staurosporine data set for which Tm estimates could be obtained. Proteins with Benjamini-Hochberg adjusted p values ≤ 0.01 are marked in red if they were exclusively found by NPARC, and in green if they were also detected by the Tm-based approach of (6). NPARC detects targets with small Tm-differences if the measurements are reproducible between replicates.