Table II. Summary of the chemical proteomics results for candidate proteins.
| Protein name | Gene names | Regulation | Relative oxidation DDW/Control | ΔTm (oC) |
|---|---|---|---|---|
| Sequestosome-1 | p62 | ↑ | 2.45 | 0.70 |
| Serine/threonine-protein kinase Chk1 | CHEK1 | ↑ | / | −0.15 |
| Cyclin-dependent kinases regulatory subunit 2 | CKS2 | ↑ | / | 0.90 |
| Gamma-aminobutyric acid receptor-associated protein-like 2 | GABARAPL2 | ↑ | / | −0.93 |
| Neuronal pentraxin-1 | NPTX1 | ↑ | / | −1.82 |
| TNF receptor-associated factor 1 | TRAF1 | ↑ | / | −0.33 |
| Calcium/calmodulin-dependent protein kinase type 1 | CAMK1 | ↑ | / | −0.40 |
| Iron-responsive element-binding protein 2 | IREB2 | ↑ | / | −1.06 |
| MORF4 family-associated protein 1 | MRFAP1 | ↑ | / | 0.58 |
| Low-density lipoprotein receptor | LDLR | ↑ | 1.02 | 0.80 |
| Kunitz-type protease inhibitor 2 | SPINT2 | ↑ | 1.33 | / |
| Kinesin-like protein KIF23 | KIF23 | ↑ | 1.52 | 1.48 |
| Hydroxymethylglutaryl-CoA synthase, cytoplasmic | HMGCS1 | ↑ | 3.64 | 0.58 |
| Calcium-binding and coiled-coil domain-containing protein 2 | CALCOCO2 | ↑ | / | −0.23 |
| Uncharacterized protein KIAA1143 | KIAA1143 | ↑ | / | −0.01 |
| Histone H2AX | H2AFX | ↑ | 1.69 | / |
| POTE ankyrin domain family member E | POTEE | ↑ | 1.38 | / |
| T-complex protein 11-like protein 2 | TCP11L2 | ↑ | / | 0.25 |
| Cell division cycle protein 20 homolog | CDC20 | ↑ | / | −0.56 |
| Heme oxygenase 1 | HMOX1 | ↑ | / | 0.10 |
| Phospholipid hydroperoxide glutathione peroxidase, mitochondrial | GPX4 | ↓ | / | 1.08 |
| Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial | ALDH4A1 | ↓ | / | −0.01 |
| NADPH:adrenodoxin oxidoreductase, mitochondrial | FDXR | ↓ | 1.66 | −0.19 |
| Ribonucleoside-diphosphate reductase subunit M2 B | RRM2B | ↓ | / | −0.21 |
| Non-histone chromosomal protein HMG-17 | HMGN2 | ↓ | / | 2.04 |
| Serum deprivation-response protein | SDPR | ↓ | / | 0.57 |
| Cyclin-dependent kinase inhibitor 1 | CDKN1A | ↓ | / | −0.54 |
| Lysosomal acid lipase/cholesteryl ester hydrolase | LIPA | ↓ | / | 1.57 |
| Carboxymethylenebutenolidase homolog | CMBL | ↓ | 0.85 | 0.44 |
| Dihydropyrimidinase-related protein 4 | DPYSL4 | ↓ | / | 0.43 |
| Sialate O-acetylesterase | SIAE | ↓ | / | −4.93 |
| Eukaryotic initiation factor 4A-II | EIF4A2 | ↓ | / | −0.71 |
| DNA damage-binding protein 2 | DDB2 | ↓ | / | −0.13 |
| Ribonucleoside-diphosphate reductase subunit M2 | RRM2 | ↓ | / | −0.21 |
| High mobility group nucleosome-binding domain-containing protein 5 | HMGN5 | ↓ | / | −0.08 |
| Glucose 1-dehydrogenase | H6PD | ↓ | / | −1.10 |
| Dihydrofolate reductase | DHFR | ↓ | / | −0.14 |
| Protein mago nashi homolog 2 | MAGOHB | ↓ | / | 0.89 |
| Thymidine kinase, cytosolic | TK1 | ↓ | / | −0.57 |
| Protein S100-A3 | S100A3 | ↓ | / | 0.92 |
| Acid ceramidase;Acid ceramidase subunit alpha; Acid ceramidase subunit beta | ASAH1 | ↓ | / | −0.03 |
| Glutathione peroxidase 2 | GPX2 | ↓ | / | −1.17 |
| Protein WWC3 | WWC3 | ↓ | / | −1.51 |
| Transcription elongation factor A protein-like 3 | TCEAL3 | ↓ | / | −0.84 |
“↑” means the protein is up-regulated (“↓” - down-regulated) in DDW specifically in respect to control drugs and NW. “/” means the protein was not identified in redox proteomics or TPP analysis.