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Overlapping peptide segmentation (OPS). Because of the partial specificity of “nonspecific” proteases like pepsin, the coverage resolution may be limited to longer peptide lengths. The resolution can be mathematically increased through Overlapping Peptide Segmentation (OPS). OPS takes advantage of peptide overlaps such as those in (A), where two peptides share exactly one terminus in common. B, shows how OPS uses such pairs to yield new peptides (C).
