Fig. 2.

GBF1 silencing reduces UUKV infection in A549 cells. A, A549 cells treated with the indicated siRNAs (20 nm) were infected with UUKV (MOI ∼1) and harvested 8 h later. Infection was analyzed by flow cytometry following immunofluorescence staining against the viral nucleoprotein N, and results were normalized to the infection of cells treated with a negative-control siRNA (si_Ctrl). Arithmetic mean ± S.E. of the mean (S.E.) from three independent infection experiments shown. B, Efficiency of GBF1 silencing assessed by Western blotting. GBF1 levels in A549 cells treated with siRNAs targeting GBF1 were compared with GBF1 levels in cells treated with negative-control siRNAs (si_Ctrl). C, A549 cells were treated with three siRNAs against GBF1 (20 nm) and exposed to UUKV (MOI ∼5). Infection was analyzed via flow cytometry, after immunofluorescence staining against N, 8 h later. The values were normalized to the infection level in samples treated with si_Ctrl. Arithmetic means ± S.E. from three independent infection experiments shown.