Fig. 3.

Accuracy, linearity and reproducibility of the 'identification' step of the method performed on the four most frequent bacteria in UTI. A, Prediction reported by the algorithm after peptidic signature monitoring by PRM associated with its probability (light blue : high probability, dark blue : low probability) for five concentrations corresponding to five inoculation volumes (1, 2, 10, 20 and 100 μl or 2, 4, 20, 40 and 200 μl) of four bacteria (Eco, Efa, Kpn or Sag) in urine of four different healthy volunteers (A, B, C, and D), dotted red line corresponds to the commonly used clinical laboratories detection threshold of 1 × 10⁵ CFU/ml; B, Linearity curves obtained for 4 peptides of the peptidic signature with the samples across the five tested concentrations, dotted red line corresponds to the commonly used clinical laboratories detection threshold of 1 × 10⁵ CFU/ml; C, Pearson correlation coefficients between two of the four biological samples (i.e. four different urines of healthy volunteers).