Fig. 4.

Accuracy, linearity and reproducibility of the 'identification step' of the method performed in two experimental conditions: 90 min gradient at nanoflow rate with PRM acquisition on an Orbitrap Fusion instrument or 30 min gradient at capillary flow rate with PRM acquisition on a Q Exactive HF-X instrument. A, Right (green) or wrong (red) prediction reported by the algorithm after peptidic signature monitoring by PRM associated to its probability, for five concentrations corresponding to five inoculation volumes (1, 2, 10, 20 and 100 μl or 2, 4, 20, 40 and 200 μl) of four bacteria (Eco, Efa, Kpn or Sag) in urine of four different healthy volunteers (A, B, C, and D), dotted red line corresponds to the commonly used clinical laboratories detection threshold of 1e5 CFU/ml; B, Distribution of the determination coefficients of the linearity curves obtained with the same samples across the five tested concentrations, the dotted line represents the average of all values; C, Distribution of the Pearson correlation coefficients obtained by comparison of two biological replicates with the same samples, the dotted line represents the average of all values.