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. 2019 Dec 2;29(6):323–334. doi: 10.1089/nat.2019.0815

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© Zhongtian Liu et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — AGO2 and TNRC6 protein cellular distribution in different cell lines. (A). Western blot of cytoplasmic and nuclear fractions from WT, AGO2^−/− and TNRC6A^−/− of calnexin, an ER protein, tubulin, a cytoplasm marker, and histone H3, a chromatin protein to show the purity of the extraction. (B) Western blot of cytoplasmic and nuclear fractions from WT, TNRC6B^−/−, and TNRC6AB^−/− showing purity of the extraction. ER, endoplasmic reticulum.