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Assay of siRNA pathways in TNRC6 knockout cell lines. (A) siRNA-mediated inhibition of nuclear noncoding RNA MALAT1 expression, n = 3. Each measurement using siMALAT1 is normalized to treatment with the corresponding siCM control RNA. (B) Western blot analysis of siRNA-mediated inhibition of ATX3 protein expression. Error bars represent standard deviation, ***P < 0.001. siCM and siGL2 are noncomplementary duplex RNAs.
