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. 2019 Nov 18;2019:4143167. doi: 10.1155/2019/4143167

Figure 4.

Figure 4

Higher BMPR1A expression was detected in ASMSCs than in HDMSCs during adipogenesis. (a) qRT-PCR was performed to detect the gene expression of BMP2, BMP4, BMP6, BMP7, BMP9, BMPR1A, BMPR1B, and BMPR2 during adipogenesis. ASMSCs (n = 25) expressed BMPR1A at higher levels than HDMSCs (n = 30) on days 7, 10, and 14. No significant difference was found between HDMSCs and ASMSCs with regard to the mRNA expression of BMP2, BMP4, BMP6, BMP7, BMP9, BMPR1B, or BMPR2. (b) ELISA was used to detect the protein expression of BMP4, BMP6, BMP7, and BMP9. The results were consistent with those of mRNA expression. (c) The protein expression of BMPR1A, BMPR1B, BMPR2, and BMP2 was determined by Western blotting. ASMSCs (n = 25) had higher BMPR1A protein expression than HDMSCs (n = 30) during adipogenic differentiation, whereas the protein expression levels of BMPR1B, BMPR2, and BMP2 were comparable between HDMSCs and ASMSCs. Values are presented as the means ± SDs. ∗P < 0.05 for the differences between HDMSCs and ASMSCs.