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. 2019 Nov 18;2019:4143167. doi: 10.1155/2019/4143167

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Copyright © 2019 Zhenhua Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lentiviruses encoding shRNA-BMPR1A had a greater inhibitory effect on adipogenic differentiation in ASMSCs than in HDMSCs. (a) The transfection and knockdown efficiencies were comparable between HDMSCs (n = 30) and ASMSCs (n = 25). (b) After transfection, ORO staining and ORO absorbance were decreased in both HDMSCs (n = 30) and ASMSCs (n = 25). However, Lv-BMPR1A exerted a stronger inhibitory effect on ASMSCs than on HDMSCs, leading to a comparable OD value between HDMSCs and ASMSCs. (c) Lv-BMPR1A inhibited expression of the PPAR-γ, FABP4, and adiponectin mRNAs in both HDMSCs (n = 30) and ASMSCs (n = 25). Lv-BMPR1A exerted a greater inhibitory effect on ASMSCs than on HDMSCs. (d) Levels of the pSmad1/5/8, PPAR-γ, FABP4, and adiponectin proteins in HDMSCs (n = 30) and ASMSCs (n = 25) were consistent with their gene expression levels. Values are presented as the means ± SDs. ^∗P < 0.05 for the differences between HDMSCs and ASMSCs.