Table 1.
Gene-specific primers used in the qPCR.
| Gene | Primer sequences(5′-3′) |
|---|---|
| IL-1β | Forward 5′-TCTCCAGCCAGTCTTCATTGT-3′ |
| Reverse 5′-GCCATCAGCCTCAAATAACAG-3′ | |
|
| |
| IL-6 | Forward 5′-AGCAAGGAGGTACTGGCAGA-3′ |
| Reverse 5′-AAGACCGGTGGTGATTCTCA-3′ | |
|
| |
| IL-10 | Forward 5′-GGGAGGATATCAAGGAGCACG-3′ |
| Reverse 5′-CTTGGAGCTTGCTAAAGGCAC-3′ | |
|
| |
| TNF-α | Forward 5′-ACCAGCCAGGAGAGAGACAAG-3′ |
| Reverse 5′-AGCGTGTGAGAGGGAGAGAGT-3′ | |
|
| |
| HGF | Forward 5′-TGATCAACTCAGACGGCCTA-3′ |
| Reverse 5′-AGCCCCAGCACATATTTCAG-3′ | |
|
| |
| Cyclin D1 | Forward 5′-AAGTGCGTGCAGAAGGAAAT-3′ |
| Reverse 5′-AGGAAGCGGTCCAGGTAGTT-3′ | |
|
| |
| TGF-β | Forward 5′-CCATTCGCGGCCAGATT-3′ |
| Reverse 5′-GCTCCGGTTCGACACTTTC-3′ | |
|
| |
| ALB | Forward 5′-TCGCCTGAGCCAGAGATTTCCC-3′ |
| Reverse 5′-CCGCCCTGTCATCTGCACATTC-3′ | |
|
| |
| HNF-4a | Forward 5′-CAAGAGGAACCAGTGCCGCTAC-3′ |
| Reverse 5′-GCTTGACCTGCGAGTGCTGATC-3′ | |
|
| |
| β-Actin | Forward 5′-TCTGGCACCACACCTTCT-3′ |
| Reverse 5′-TGATCTGGGTCATCTTCTCAC-3′ | |
IL-1β: interleukin-1 beta; IL-6: interleukin-6; IL-10: interleukin-10; TNF-α: tumor necrosis factor alpha; HGF: hepatocyte growth factor; TGF-β: transforming growth factor-beta; ALB: albumin; HNF-4a: hepatocyte nuclear factor 4a.