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. 2019 Nov 18;2019:3585390. doi: 10.1155/2019/3585390

Figure 4.

Figure 4

Myomaker expression depends on Nox4-mediated ROS. After C2C12 cells were treated with either siCont or siNox4, mRNA levels of Nox4 (a) and Mymk (b) during differentiation were determined by RT-qPCR, using 36B4 for normalization.∗∗p < 0.01, #p < 0.001 (n = 3). (c) Protein levels of Nox4 and myomaker were determined by immunoblotting. Following pretreatment with siNox4, GKT137831 (GKT), or NAC in growth medium, C2C12 cells were incubated in DM for 2 days, ROS production was evaluated by DCF-DA assay (d), the Mymk mRNA level was determined by RT-qPCR using 36B4 for normalization (e), and protein levels of Nox4 and myomaker were estimated by immunoblotting (f). Overexpression of Nox4 was achieved by infection with Ad-Nox4 (MOI = 10). ∗p < 0.05, ∗∗p < 0.01, #p < 0.001 (n = 3).