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. 2019 Nov 18;2019:6565283. doi: 10.1155/2019/6565283

Figure 5.

Figure 5

Methane-rich saline downregulated the expression of the TLR/NF-κB signaling pathway. MRS treatment in rats was achieved by intraperitoneal administration (10 mL/kg) for 7 days after BDL. (a) Immunoblot assays of TLR4, Myd88, and NF-κB (p65). Relative densities of (b–d) TLR4, Myd88, and NF-κB (p65). (e) Immunofluorescence staining of TLR4. (f) The percentage of TLR4-positive cells. (g) Immunofluorescence staining of NF-κB (p65). (h) The percentage of NF-κB (p65)-positive cells. (n = 10, scale bars: 25 μm. Data are shown as the mean ± SD. ∗p < 0.05 versus the sham control group; #p < 0.01 versus the sham control group; €p < 0.05 versus the MRS control group; $p < 0.01 versus the MRS control group; &p < 0.05 versus the BDL+NS group; γp < 0.01 versus the BDL+NS group).