Figure 4.

Effects of GSPB2 on the H₂O₂-induced downregulation of let-7a expression in GCs. (a) The expression levels of let-7a in cultured GCs were measured after 6 h of exposure to 0, 50, 100, 200, 300, and 500 μmol/L H₂O₂. (b) GCs were pretreated with GSPB2 (10 μmol/L) for 24 h followed by H₂O₂ (200 μmol/L) for 6 h. The mRNA expression level of let-7a was analyzed by RT-PCR. Each value is expressed as the mean ± S.E. (n = 3). ^#P < 0.05 H₂O₂ alone group vs. the control; ^∗P < 0.05 vs. the H₂O₂ alone group.