Figure 5.

Effect of let-7a on GC apoptosis and caspase-3 activity. GCs were transfected with let-7a mimics and/or pretreated with GSPB2 and subsequently treated with H₂O₂ (200 μmol/L) for 6 h. (a) The number of apoptotic cells was identified by the TUNEL method. (b) The levels of caspase-3 activity were measured by ELISA. GCs were transfected with let-7a inhibitors and/or pretreated with GSPB2 and subsequently treated with H₂O₂ (200 μmol/L) for 6 h. (c) The apoptosis number in different groups was determined by the TUNEL method. (d) The caspase-3 activity in different groups was measured by ELISA. Each value is expressed as the mean ± S.E. (n = 3). ^∗P < 0.05 vs. the control; ^#P < 0.05 vs. the antioxidant pretreatment; ⁺P < 0.05 vs. H₂O₂; ^&P < 0.05 vs. the GSPB2 pretreatment and H₂O₂.