Figure 7.

let-7a binds to the 3′-UTR of Fas and regulates gene expression. (a) Pathway enrichment analysis of let-7a targets. (b) The putative let-7a-binding sites in the porcine Fas 3′-UTR (red letters). The blue letters show the region that was mutated in the experiment shown in (b). (c) A dual-luciferase reporter assay was performed by cotransfection of HEK293T with luciferase reporters containing a WT or mutant 3′-UTR of porcine Fas with a let-7a mimic. (d) The Fas protein expression level was normalized to that of β-actin. Each value is expressed as the mean ± S.E. (n = 3). ^∗P < 0.05, ^∗∗P < 0.01 vs. the control group.