Figure 4.

Biodistribution of intraperitoneally injected DFP‐10825 in a MKN45 peritoneally disseminated mouse model. MKN45 peritoneally disseminated model mice were intraperitoneally injected with free Alexa750‐labeled TS short hairpin RNA (shRNA), DFP‐10825 containing Alexa750‐labeled TS shRNA, or DFP‐10825 containing 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethylindotricarbocyanine iodide (DiR) (20 µg thymidylate synthase [TS] shRNA/mouse). A, The fluorescence of Alexa750‐labeled TS shRNA or DiR in the formulation was monitored with in vivo imaging system (IVIS) at selected time points (5, 10, and 30 min; 1, 3, 6, 12, 24, 48, and 72 h post‐injection). B, After preserving an image at 72 h post‐injection, tumors (Tu) and organs including heart (He), lung (Lu), liver (Li), spleen (Sp), and kidney (Ki) were harvested. The fluorescence of Alexa750‐labeled TS shRNA or DiR in each of the tissues was visualized with IVIS