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. 2019 Oct 7;8(17):7265–7277. doi: 10.1002/cam4.2594

Figure 3.

Figure 3

The effect of propranolol on apoptotic cell death and cell cycle arrest in melanoma cells. TUNEL staining showed the presence of apoptotic cells under 50, 100, 150, and 200 μmol/L propranolol exposure during 24 h in (A) uveal melanoma (UM) (Mel 270 and MP41) and (B) cutaneous melanoma (CM) (WM266.4) cells. Cells under DNase were used as a positive control. Green color indicates TUNEL‐positivity, blue color marks nucleus. 20× objective. Representative flow cytometry analysis of UM using Annexin V FITC (C). Bar graph of early apoptosis, necrosis, and cell death on cultured UM cells (D). Cell cycle analysis was done by flow cytometry following propranolol exposure at 6 concentrations in UM (E) and CM (F) cell lines. Error bars represent ± 1 SD. *P < .05 vs control (0 μmol/L). cell‐free DNA (cfDNA) in the supernatant was assessed following 24 h treatment with propranolol in (G) UM (Mel 270 and OMM2.5) and (H) CM (WM266.4 and WM115) cell lines. The specific mutation for each cell line is indicated. *P < .05 vs control (0 μmol/L)