Figure 3.

Zinc ribbon domain‐containing 1 (ZNRD1) silencing inhibits hepatocellular carcinoma (HCC) cell proliferation, migration, and invasion. A, HCC cells SMMC‐7721 or Hep 3B were transfected with negative control (NC) or different shRNAs targeting ZNRD1. The knockdown efficiency was analyzed by western blotting 48 h later. GAPDH was used as a loading control. B, Effects of ZNRD1 knockdown on cell proliferation were determined by CCK‐8 cell proliferation assays. Data were represented as the means ± SD. C, DNA synthesis in HCC cells SMMC‐7721 or Hep 3B was measured using the EdU incorporation assay. D, Effects of ZNRD1 knockdown on cell proliferation were determined by colony formation assay. E, Transwell assays were performed to evaluate the effect of ZNRD1 knockdown on cell invasion capability. Cells were counted under a microscope in five randomly selected fields. F, Wound‐healing assays were performed to evaluate the effect of ZNRD1 knockdown on cell migration. *P < .05, **P < .01