Figure 4.

Zinc ribbon domain‐containing 1 (ZNRD1) silencing suppresses tumorigenesis in nude mice. Hepatocellular carcinoma SMMC‐7721 cells transfected with Ctrl or sh‐ZNRD1 were implanted subcutaneously into nude mice and tumor growth was monitored. A, Representative photos of nude mice and colon tumor tissues from Ctrl or sh‐ZNRD1 group at week 5. B, Growth curves of subcutaneous xenografts were determined based on tumor size measured every week. C, Relative photon flux of tumors in Ctrl or sh‐ZNRD1 group was determined by quantifying the bioluminescent signal. D, Tumor weight were measured and represented as means of tumor weights ± SD. E, The representative images of H&E staining, ZNRD1 and Ki‐67 IHC staining of tumor sections from Ctrl or sh‐ZNRD1 group. F and G, Quantification of relative ZNRD1 and Ki‐67 staining intensity in tumor sections from the Ctrl or sh‐ZNRD1 group. *P < .05, **P < .01