Figure 5.

Zinc ribbon domain‐containing 1 (ZNRD1) activates Wnt/β‐catenin signaling pathway in hepatocellular carcinoma (HCC). A, GSVA analysis showed that Wnt/β‐catenin pathway in tumor compared with adjacent nontumor were identified as the top three activated regulated pathway in TCGA HCC cancer cohort. B, The Gene Set Enrichment Analysis of the correlation between ZNRD1 expression and gene signatures of Wnt/β‐catenin signaling pathway in HCC. C and D, SMMC‐7721 or Hep 3B cells were transfected with Ctrl or sh‐CLK3 and the expression of Wnt3a, β‐catenin, APC, and cyclinD1 was analyzed by western blot. Relative quantification analysis was based on grayscale values. *P < .05, **P < .01