Figure 7.

MiR‐26b inhibits hepatocellular carcinoma cell proliferation, invasion and migration in vitro by targeting Zinc ribbon domain‐containing 1 (ZNRD1). SMMC‐7721 or Hep 3B cells were transfected with NC, miR‐26b mimics or miR‐26b mimics & ZNRD1 overexpression plasmid. A, The expression levels of ZNRD1, Wnt3a, β‐catenin, APC, and cyclinD1 were analyzed by western blot 48 h later. B, Cell proliferation was determined by CCK‐8 cell proliferation assays. C, Colonies of SMMC‐7721 or Hep 3B in different groups were determined by colony formation assay. D, Cell invasion capability of SMMC‐7721 or Hep 3B in different groups was analyzed by transwell assay. E, Cell migration capability of SMMC‐7721 or Hep 3B cells in different groups was analyzed by wound‐healing assay. *P < .05, **P < .01