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MCF-7-R cells exhibited stronger stemness than did MCF-7 cells. (A) ALDH1 activity was examined in MCF-7-R and MCF-7 cells. (B, C) The spheroid forming ability was evaluated in MCF-7-R and MCF-7 cells at various dilutions. (D, E) QRT-PCR and western blot analysis of the expression of critical stemness regulators in MCF-7-R and MCF-7 cells. ** p<0.01 vs. MCF-7.
