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NP reduced the stemness of MCF-7-R cells. (A) Analysis of ALDH activity in MCF-7-R cells treated with different concentrations of NP. (B, C) Analysis of spheroid formation ability was performed in MCF-7-R cells treated with different concentrations of NP. (D, E) Western blot analysis of the expression of critical stemness regulators was carried out in MCF-7-R cells treated with different concentrations of NP. * p<0.05, ** p<0.01 vs. control.
