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. 2019 Nov 24;25:8905–8912. doi: 10.12659/MSM.918384

Figure 4.

Figure 4

NP attenuated MCF-7-R cell stemness through suppressing STAT3 activation. (A) The activity of pGL4.47 was measured in MCF-7-R and MCF-7 cells by luciferase reporter analysis. (B) The activity of pGL4.47 was assessed in MCF-7-R cells with or without NP treatment. (C) The expression of p-STAT3 was detected in MCF-7-R cells after treatment with different concentrations of NP. (D) Total STAT3 expression was examined in MCF-7-R cells with NP, IFN-γ, or NP plus IFN-γ treatment. (E) The expression of the downstream effectors of IFN-γ (c-Myc and Bcl-xl) was determined in MCF-7-R cells after treatment with different concentrations of NP. (F) The expression of total STAT3 was measured in MCF-7-R cells treated with NP, transfected with pc-STAT3, or with both. (G) Analysis of ALDH1 activity was performed in MCF-7-R cells treated with NP, transfected with pc-STAT3, or with both. (H, I) Spheroid forming ability was evaluated in MCF-7-R cells treated with NP, transfected with pc-STAT3, or with both. (J) The mRNA levels of stemness critical regulators were examined in MCF-7-R cells treated with NP, transfected with pc-STAT3, or with both. * p<0.05, ** p<0.01 vs. control.