Figure 1.

NGF defends against Aβ25–35‐triggered neuronal toxicity in SKNSH cells. (A) Twenty‐four‐hour incubation was conducted using various Aβ25–35 concentrations. Scale bars: 100 nm. (B) Cells received supplementation with Aβ25–35 (25 μm) for the indicated time. (C) Cells received supplementation with NGF at the particular concentrations for 24 h. (D, E) Cells received preliminary NGF supplementation (0, 25, 50, or 100 ng·mL⁻¹) for 24 h before the addition of 25 μm Aβ25–35. Survival was evaluated using a CCK‐8 assay in SKNSH cells (D) and primary neurons (E). Results are expressed as mean ± SEM for three independent experiments. One‐way ANOVA, *P < 0.05, **P < 0.01 vs. control; ^# P < 0.05, ^## P < 0.01 vs. Aβ25–35 alone.