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. 2019 Sep 30;9(12):2025–2040. doi: 10.1002/2211-5463.12650

Figure 6.

Figure 6

(A) Interactions of MurA with other proteins identified using STRING v.10.0 database. MurA, involved in catalyzing the first step in peptidoglycan biosynthesis, displayed strong interactions with proteins regulating peptidoglycan and cell‐wall biogenesis. (B) Western blot analysis of MurA. The alteration of MurA protein in response to UA–norfloxacin treatment was validated using western blotting, which confirmed the down‐regulation of MurA, indicated by lowered band width and intensity. GAPDH, an endogenous control, was used as loading control in the experiment. (C,D) Quantitative real‐time PCR analysis relative mRNA expression levels of genes up‐regulated in MRSA‐2071 (C) and down‐regulated (D) under UA–norfloxacin treatment alone and in combination using RT‐PCR. The relative expression analysis of each gene was carried out by normalizing the data with GAPDH as an endogenous control and the error bars represent standard deviation (n = 3).