Table 3.

List of differentially expressed proteins in methicillin resistant Staphylococcus aureus SA‐2071 upon UA–norfloxacin exposure at sub‐lethal concentrations obtained from two‐dimensional electrophoresis analysis. D, down‐regulated; U, up‐regulated

Spot no.	Name of protein	Uniprot ID	Gene	M (Da)	No. of peptides	Fold change	Score	P‐value
U1	Superoxide dismutase (Mn/Fe)	P99098	sodA	22 711	12	0.27	243	0.004
U2	Alkyl hydroperoxide reductase subunit C	P99074	ahpC	20 977	8	0.33	217	0.005
U3	Enolase	P99088	eno	47 117	23	0.29	332	0.008
U4	Catalase	Q7A5T2	katA	58 380	27	0.23	207	0.009
U5	Probable quinol oxidase subunit 2	Q7A698	qoxA	41 777	24	0.31	199	0.016
U6	Probable thiol peroxidase	P99146	tpx	18 019	6	0.41	151	0.024
U7	Bacterial non‐heme ferritin	Q7A4R2	ftnA	19 589	5	0.49	148	0.027
U8	Protein GrpE	P99086	grpE	24 008	10	0.61	97	0.001
U9	Iron–sulfur cluster repair protein ScdA	Q7A7U6	scdA	25 485	17	0.35	226	0.019
U10	Transketolase	P99161	tkt	72 317	22	0.24	316	0.013
U11	Chaperone Protein DnaK	P99110	dnaK	66 361	20	0.35	208	0.024
U12	Peroxide responsive repressor (PerR)	Q7A4T8	perR	17 183	4	0.37	114	0.003
U13	UPF0413 protein SAUSA300_0983	Q2FI75	SAUSA300_0903	31 364	12	0.29	328	0.004
U14	Putative 2‐hydroxyacid dehydrogenase SAR2389	Q6GEC9	SAR2389	34 675	15	0.15	175	0.004
D1	Fructose‐bisphosphate aldolase Class I	P99117	fda	32 913	13	1.37	266	0.043
D2	d‐Lactate dehydrogenase	P99116	ldhD	36 756	18	0.95	254	0.036
D3	LipidII: glycine glycyltransferase	Q7A447	femX	48 513	21	1.48	310	0.001
D4	Putative antiporter subunit nnhG2	Q7A722	mnhG2	16 371	9	1.22	163	0.027
D5	DNA translocase FtsK	P64165	ftsK	90 682	26	1.06	336	0.013
D6	Trigger factor	P99080	tig	48 609	15	1.69	154	0.032
D7	UDP‐N‐acetylmuramoyl tripeptide–d‐alanyl‐d‐alanine ligase	A0A0H3JMW3	murF	50 065	8	3.53	186	0.007
D8	Enoyl‐(acyl‐carrier‐protein) reductase (NADPH)	A0A0H3JLH9	fabI	27 992	4	1.98	119	0.004
D9	3‐Oxoacyl‐(acyl‐carrier‐protein) synthase 3	P99159	fabH	33 879	11	1.96	176	0.023
D10	30S Ribosomal protein S12	P0A0G8	rpsL	15 287	9	1.88	143	0.018
D11	Uncharacterized lipoprotein SAUSA300_0411	Q2FJK3	SAUSA300_0411	30 917	10	1.52	281	0.047
D12	Tetracycline resistance protein tetM from transposon Tn5251	Q54807	tetM(5251)	72 556	16	2.15	294	0.024
D13	Deoxyribose phosphate aldolase 2	P99174	deoC2	23 218	14	1.36	173	0.022
D14	Formate acetyltransferase	Q7A7X6	pflB	84 862	23	1.73	304	0.021
D15	Adenine phosphoribosyltransferase	P68779	apt	19 117	12	1.84	265	0.03
D16	Fructose‐bisphosphate aldolase Class I	P99117	fda	32 913	16	1.34	208	0.005
D17	Fructose‐bisphosphate aldolase Class II	P99075	fba	30 836	18	1.42	314	0.014
D18	ATP synthase subunit c	Q7A4E6	atpE	6979	3	1.26	101	0.029
D19	HTH‐type transcriptional regulator SarR	Q7A425	sarR	13 669	5	1.9	151	0.006
D20	UDP‐N‐acetylglucosamine 1‐carboxyvinyltransferase	P84058	murA	45 054	15	6.64	163	.012
1a	60 kDa chaperonin	P99083	groEL	57 630	13	n/a	138	0.034
2a	General stress protein	W8U554	yugI_1	14 723	7	n/a	127	0.005
1b	50S ribosomal protein L17	Q7A469	rplQ	13 529	5	n/a	134	0.014
1c	DEAD‐box ATP‐dependent RNA helicase CshA	Q7A4G0	cshA	56 942	20	0	193	0.023
2c	Transcriptional regulatory protein WalR	Q7A8E1	walR	27 192	13	0	269	0.016
3c	Elongation factor Tu	P99152	tuf	43 104	15	0	338	0.018
4c	UPF0355 protein MRSA252	Q6GJR0	SAR0405	15 051	6	0	193	0.026

^a Uniquely expressed in only UA‐treated cells. ^b Uniquely identified in norfloxacin‐exposed cells. ^c Unique in UA–norfloxacin‐treated cells).