Figure 2.

The levels of cytokines producing by different subtypes of monocyte from PBMCs of patients in vitro. (A) The sorting strategy for the subtypes of monocytes from PBMCs of patients with sepsis. The CD14²⁺CD16⁻, CD14⁺CD16⁺ intermediate and CD14^lowCD16²⁺ monocytes were isolated from CD16⁺CD14⁺ monocytes according to flow sorting. (B) The levels of inflammatory cytokines producing by PBMCs from patients with a higher or lower level of TSLP in cultural supernatant after stimulation with lipopolysaccharide (LPS). (C) The levels of inflammatory cytokines producing by CD14^lowCD16²⁺ nonclassical monocytes from patients with a higher or lower level of TSLP in cultural supernatant after stimulation with LPS. (D) The levels of inflammatory cytokines producing by CD14²⁺CD16⁻ nonclassical monocytes from patients with a higher or lower level of TSLP in cultural supernatant after stimulation with LPS. Data are presented by mean ± SD (error bar). The unpaired Student’s t‐test was used for statistical analysis. The two‐sided test was applied. P < 0.05 indicated statistical significance: **P < 0.01. n = 10. HLA, human leukocyte antigen.