Figure 1.

Fd‐affinity chromatography of the stroma fraction of spinach chloroplasts (A) and western blot analysis of the elution fractions using anti‐L‐FNR antibody (B). The chromatography was performed in the absence (control) and in the presence of NADP⁺ or NAD⁺ at 1 mm using maize Fd I‐immobilized resin (1 mL volume) developed with 0–300 mm NaCl in 50 mm Tris/HCl, pH 7.5. Fifteen microliters out of 500 μL of each twenty fractions (depicted in panel A and B) was loaded on the SDS/PAGE gel. Panel A shows the chromatography patterns in the absence (control) and in the presence of NADP⁺ and NAD⁺, monitored with absorbance at 280 nm.