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. 2019 Nov 20;2019:8419493. doi: 10.1155/2019/8419493

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Copyright © 2019 Tomoko Hyakumura et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Stem cell-derived neurons innervated the edge of CN slices within 14 days. Stem cell-derived neurons were cocultured with rat CN slices with the VIII nerve (ventral side) facing towards them (a). Within 14 days of culture, stem cell-derived neurons extensively innervated the edge of CN slices in cocultures (b, red square). These stem cell-derived neurites (positive for human NFM (red) and NFH (blue)) (c–f) innervated the edge of the CN slices and were immunopositive for synaptic markers synapsin I (c, d) and VGLUT1 (e, f). Scale bar in (f) = 20 μm, applies to all panels (c)–(f).