Table 1.
Whole explant control | HC-only control | AN/HC control | SC/HC coculture | |
---|---|---|---|---|
Preparation | Whole cochlear explant where HCs and ANs are kept intact | HC-only explant where ANs are dissected from HCs | HC rows and ANs are separated and cocultured | HCs and SC-derived neurons: hair cell rows are cocultured with SC-derived neural progenitors |
Culture period | 1 DIV | 12-14 DIV | 12-14 DIV | 12-14 DIV |
Images | ||||
Controls for | Positive control for synapses between HCs and endogenous ANs | Negative control for synapses | For comparison between SC-derived neurons and endogenous ANs in reinnervation | Experimental group |
HC: hair cell; AN: auditory neuron; SC: stem cell; DIV: days in vitro.