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. 2019 Nov 20;2019:8419493. doi: 10.1155/2019/8419493

Table 2.

Summary of experimental groups for central innervation cocultures.

CN control SC control SC/CN coculture
Preparation Cochlear nucleus slice (200 μm thick) Human PSC-derived neurons cultured on their own Human PSC-derived neurons cultured with cochlear nucleus slice
Culture period 1 or 14 DIV 14 DIV 14 DIV
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Controls for The presence of VGLUT1 in cultured CN slice without addition of hPSC The presence of VGLUT1 in cultured hPSC without CN The specificity of hPSC-derived neural innervation

CN: cochlear nucleus; SC: stem cell; DIV: days in vitro.