Antibodies |
|
Rabbit polyclonal to human GATA1 |
Santa Cruz Biotechnology |
Cat#sc1234, RRID:AB_2263157 |
Rabbit mAb to human GATA1 |
Cell Signaling |
Cat#3535, RRID:AB_2108288 |
Rabbit polyclonal to CASP1 |
Santa Cruz Biotechnology |
Cat#sc56036, RRID:AB_781816 |
Rabbit polyclonal to zebrafish Gatal a |
GeneTex |
Cat#GTX128333 |
Rabbit polyclonal to zebrafish Spi1 b |
GeneTex |
Cat#GTX128266 |
Rabbit polyclonal to histone H3 |
Abcam |
Cat#ab1791, RRID:AB_302613 |
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse |
Sigma-Aldrich |
Cat#A8592, RRID:AB_439702 |
Streptavidin-BV711 |
BD Biosciences |
Cat#563262 |
anti-Sca1 conjugated with BV510 |
Biolegend |
Cat#108129, RRID:AB_2561593 |
anti-cKIT conjudated with BV421 |
Biolegend |
Cat#105828, RRID:AB_11204256 |
anti-CD135 conjugated with PerCPeFL710 |
Thermo Fisher Scientific |
Cat#46-1351-82, RRID:AB_10733393 |
anti-CD34 conjugated wth eFL660 |
Thermo Fisher Scientific |
Cat#50-0341-82, RRID:AB_10596826 |
anti-CD48 conjugated wth APCeFL780 |
Thermo Fisher Scientific |
Cat#47-0481-82, RRID:AB_2573962 |
anti-CD150 conjugated with BV650 |
Biolegend |
Cat#115932, RRID:AB_2715765 |
anti-B220-biotin |
Thermo Fisher Scientific |
Cat#13-0452-86, RRID:AB_466451 |
anti-CD19-biotin |
Thermo Fisher Scientific |
Cat#13-0191-86, RRID:AB_466386 |
antiCd3e-biotin |
Thermo Fisher Scientific |
Cat#13-0031-85, RRID:AB_466320 |
anti-CD11b-biotin |
Thermo Fisher Scientific |
Cat#13-0112-85, RRID:AB_466360 |
anti-Gr1-biotin |
Thermo Fisher Scientific |
Cat#13-5931-85, RRID:AB_466801 |
anti-Ter119-biotin |
Thermo Fisher Scientific |
Cat#13-5921-85, RRID:AB_466798 |
anti-CD41-APC |
Thermo Fisher Scientific |
Cat#17-0411-82, RRID:AB_1603237 |
anti-CD16/32-BV421 |
Biolegend |
Cat#101332, RRID:AB_2650889 |
|
Bacterial and Virus Strains |
|
Salmonella enterica serovar Typhimurium, strain 12023 (wild type) |
Prof. David Holden |
|
|
Chemicals, Peptides, and Recombinant Proteins |
|
EnGen® Cas9 NLS from Streptococcus pyogenes
|
New England Biolabs |
Cat#M0646 |
Alt-R® CRISPR-Cas9 tracrRNA |
Integrated DNA Technologies |
Cat#1072532 |
Alt-R® CRISPR-Cas9 Negative Control crRNA |
Integrated DNA Technologies |
Cat#1072544 |
Ac-YVAD-CMK |
Peptanova |
Cat#3180-v |
Ac-YVAD-CHO |
Peptanova |
Cat#3165-v |
Ac-LEVD-CHO |
Peptanova |
Cat#864-42-v |
Metronidazole |
Sigma Aldrich |
Cat#M1547 |
Sudan black |
Sigma-Aldrich |
Cat#380B-1KT |
Z-YVAD-AFC |
Merck |
Cat#688225 |
ANTIFLAG® M2 |
Sigma-Aldrich |
Cat#A2220 |
Recombinant caspase-1 |
GeneTex |
Cat#GTX65025 |
4–15% SDS-PAGE |
BioRad |
Cat#456-1084 |
DNase I, amplification grade |
ThermoFisher Scientific |
Cat#18068015 |
SuperScript III RNase H− Reverse Transcriptase |
ThermoFisher Scientific |
Cat#18080085 |
Power SYBR Green Master Mix |
ThermoFisher Scientific |
Cat#4368708 |
Liberase TM research Grade |
Sigma-Aldrich |
Cat#05401119001 |
Protease inhibitor cocktail |
Sigma-Aldrich |
Cat#P8340 |
Hemin |
Sigma-Aldrich |
Cat#16009-13-5 |
ACK lysing buffer |
Lonza |
Cat#10-548E |
RPMI-1640 |
Sigma-Aldrich |
Cat#R0883 |
FCS |
Cultek |
Cat#91S181 D-050 |
Penicillin/Streptomycin |
Sigma-Aldrich |
Cat#P4333 |
Glutamine |
Sigma-Aldrich |
Cat#G7513 |
IMDM |
ThermoFisher Scientific |
Cat# 21056023 |
BIT |
Stem Cell Technologies |
Cat# 09500 |
IL3 |
Peprotech |
Cat#213–13 |
IL6 |
Peprotech |
Cat#216–16 |
SCF |
Peprotech |
Cat#250–03 |
EPO |
Peprotech |
Cat#100–65 |
TPO |
Peprotech |
Cat#315–14 |
|
Experimental Models: Cell Lines |
|
K562 |
ATCC |
Cat#CCL243 |
|
Experimental Models: Organisms/Strains |
|
Zebrafish casper line (roya9/a9; nacrew2/w2) |
Prof. LI Zon |
|
Tg(mpx:eGFP)i114
|
Prof. SA Renshaw |
|
T9(mpe91:eGFP)gl22
|
Prof. G Lieschke |
|
T9(mpeg1:GAL4)9125
|
Prof. G Lieschke |
|
Tg(lyz:dsRED)nz50
|
Prof. P Crosier |
|
T9(mpx:Gal4.VP16)i222
|
Prof. SA Renshaw |
|
T9(lcr:eGFP)cz3325
|
Prof. LI Zon |
|
T9(runx1:GAL4)utn6
|
Prof. LI Zon |
|
T9(UAS:nfsB-mCherry)c264
|
Prof. M. Halpern |
|
T9(spint1a)hi2217
|
Prof. M. Hammerschmidt |
|
SPI1-eYFP/GATA1-mCherry1 reporter mice (C57BL/6J background) |
Prof. Timm Schroeder |
|
|
Recombinant DNA |
|
Tol2kit |
Dr. K. Kwan |
http://tol2kit.genetics.utah.edu/index.php/Main_Page |