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. Author manuscript; available in PMC: 2019 Dec 3.
Published in final edited form as: Immunity. 2019 Jun 4;51(1):50–63.e5. doi: 10.1016/j.immuni.2019.05.005

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER

Antibodies

Rabbit polyclonal to human GATA1 Santa Cruz Biotechnology Cat#sc1234, RRID:AB_2263157
Rabbit mAb to human GATA1 Cell Signaling Cat#3535, RRID:AB_2108288
Rabbit polyclonal to CASP1 Santa Cruz Biotechnology Cat#sc56036, RRID:AB_781816
Rabbit polyclonal to zebrafish Gatal a GeneTex Cat#GTX128333
Rabbit polyclonal to zebrafish Spi1 b GeneTex Cat#GTX128266
Rabbit polyclonal to histone H3 Abcam Cat#ab1791, RRID:AB_302613
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse Sigma-Aldrich Cat#A8592, RRID:AB_439702
Streptavidin-BV711 BD Biosciences Cat#563262
anti-Sca1 conjugated with BV510 Biolegend Cat#108129, RRID:AB_2561593
anti-cKIT conjudated with BV421 Biolegend Cat#105828, RRID:AB_11204256
anti-CD135 conjugated with PerCPeFL710 Thermo Fisher Scientific Cat#46-1351-82, RRID:AB_10733393
anti-CD34 conjugated wth eFL660 Thermo Fisher Scientific Cat#50-0341-82, RRID:AB_10596826
anti-CD48 conjugated wth APCeFL780 Thermo Fisher Scientific Cat#47-0481-82, RRID:AB_2573962
anti-CD150 conjugated with BV650 Biolegend Cat#115932, RRID:AB_2715765
anti-B220-biotin Thermo Fisher Scientific Cat#13-0452-86, RRID:AB_466451
anti-CD19-biotin Thermo Fisher Scientific Cat#13-0191-86, RRID:AB_466386
antiCd3e-biotin Thermo Fisher Scientific Cat#13-0031-85, RRID:AB_466320
anti-CD11b-biotin Thermo Fisher Scientific Cat#13-0112-85, RRID:AB_466360
anti-Gr1-biotin Thermo Fisher Scientific Cat#13-5931-85, RRID:AB_466801
anti-Ter119-biotin Thermo Fisher Scientific Cat#13-5921-85, RRID:AB_466798
anti-CD41-APC Thermo Fisher Scientific Cat#17-0411-82, RRID:AB_1603237
anti-CD16/32-BV421 Biolegend Cat#101332, RRID:AB_2650889

Bacterial and Virus Strains

Salmonella enterica serovar Typhimurium, strain 12023 (wild type) Prof. David Holden

Chemicals, Peptides, and Recombinant Proteins

EnGen® Cas9 NLS from Streptococcus pyogenes New England Biolabs Cat#M0646
Alt-R® CRISPR-Cas9 tracrRNA Integrated DNA Technologies Cat#1072532
Alt-R® CRISPR-Cas9 Negative Control crRNA Integrated DNA Technologies Cat#1072544
Ac-YVAD-CMK Peptanova Cat#3180-v
Ac-YVAD-CHO Peptanova Cat#3165-v
Ac-LEVD-CHO Peptanova Cat#864-42-v
Metronidazole Sigma Aldrich Cat#M1547
Sudan black Sigma-Aldrich Cat#380B-1KT
Z-YVAD-AFC Merck Cat#688225
ANTIFLAG® M2 Sigma-Aldrich Cat#A2220
Recombinant caspase-1 GeneTex Cat#GTX65025
4–15% SDS-PAGE BioRad Cat#456-1084
DNase I, amplification grade ThermoFisher Scientific Cat#18068015
SuperScript III RNase H Reverse Transcriptase ThermoFisher Scientific Cat#18080085
Power SYBR Green Master Mix ThermoFisher Scientific Cat#4368708
Liberase TM research Grade Sigma-Aldrich Cat#05401119001
Protease inhibitor cocktail Sigma-Aldrich Cat#P8340
Hemin Sigma-Aldrich Cat#16009-13-5
ACK lysing buffer Lonza Cat#10-548E
RPMI-1640 Sigma-Aldrich Cat#R0883
FCS Cultek Cat#91S181 D-050
Penicillin/Streptomycin Sigma-Aldrich Cat#P4333
Glutamine Sigma-Aldrich Cat#G7513
IMDM ThermoFisher Scientific Cat# 21056023
BIT Stem Cell Technologies Cat# 09500
IL3 Peprotech Cat#213–13
IL6 Peprotech Cat#216–16
SCF Peprotech Cat#250–03
EPO Peprotech Cat#100–65
TPO Peprotech Cat#315–14

Experimental Models: Cell Lines

K562 ATCC Cat#CCL243

Experimental Models: Organisms/Strains

Zebrafish casper line (roya9/a9; nacrew2/w2) Prof. LI Zon
Tg(mpx:eGFP)i114 Prof. SA Renshaw
T9(mpe91:eGFP)gl22 Prof. G Lieschke
T9(mpeg1:GAL4)9125 Prof. G Lieschke
Tg(lyz:dsRED)nz50 Prof. P Crosier
T9(mpx:Gal4.VP16)i222 Prof. SA Renshaw
T9(lcr:eGFP)cz3325 Prof. LI Zon
T9(runx1:GAL4)utn6 Prof. LI Zon
T9(UAS:nfsB-mCherry)c264 Prof. M. Halpern
T9(spint1a)hi2217 Prof. M. Hammerschmidt
SPI1-eYFP/GATA1-mCherry1 reporter mice (C57BL/6J background) Prof. Timm Schroeder

Recombinant DNA

Tol2kit Dr. K. Kwan http://tol2kit.genetics.utah.edu/index.php/Main_Page