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. 2019 Nov 22;10:819. doi: 10.3389/fendo.2019.00819

Figure 1.

Figure 1

Synaptosomal densities of AdipoR1 and AdipoR2. (A) Representative immunoblot showing AdipoR1 immunoreactivity normalized to beta actin in hippocampal fractions. (B) Representative immunoblot showing AdipoR2 immunoreactivity normalized to beta actin in hippocampal fractions. Hippocampal lysate was divided into total (Total) and synaptosomal (Syn) fractions. The synaptosome was further fractionated into the PSD (Post) and non-PSD (Pre) fractions. Synaptic fractionation efficiency is represented by immunoreactivity of PSD95 and SNAP25 for Post and Pre, respectively. Twenty five micrograms of protein were loaded per lane. Bars represent mean ± SEM from 3 independent experiments, hippocampi from 2 mice were pooled for each experiment (n = 6 mice); *indicates significant difference vs. Total; #indicates significant difference vs. Post; *p < 0.05, **p < 0.01, ###p < 0.001. Tukey's post hoc test was used for multiple comparisons.