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. 2019 Nov 22;10:819. doi: 10.3389/fendo.2019.00819

Figure 3.

Figure 3

APN-KO mice display alterations in basal synaptic transmission and paired pulse facilitation which are rescued through incubation with AdipoRon. For (A–D), hippocampal slices were prepared from Control and APN-KO mice and incubated for 2-h in either ACSF containing 0.03% DMSO [Vehicle (V)] or ACSF containing 15 μM of AdipoRon and 0.03% DMSO [AdipoRon (AR)] prior to recording. (A) Input-output curve of fEPSP slope measured at increasing stimulus intensities. (B) Input-output curve of FV amplitude measured at increasing stimulus intensities. (C) Slope of the linear regression line of best fit from plotting fEPSP slope vs. FV amplitude. (D) Paired-pulse facilitation expressed as the change in ratio of the second stimulus fEPSP to the first stimulus fEPSP slope plotted as a function of interstimulus interval. (E) Representative immunoblot showing vGLUT1 and SNAP25 relative densities normalized to beta actin in total hippocampal lysate. Forty micrograms of protein were loaded per lane. Symbols/bars represent mean ± SEM; *indicates significant difference between APN-KO and Control, #indicates significant difference between APN-KO and APN-KO + AR; */#p < 0.05, **/##p < 0.01, ***p < 0.001; for (A–D), n = 5–6 slices from 4 mice per group; for (E), n = 4 mice per group; for (A–C), Tukey's post hoc test was used for multiple comparisons; for (D), planned pairwise comparisons were performed for individual data point analysis for Control vs. APN-KO, APN-KO vs. APN-KO + AR, and Control vs. APN-KO + AR.