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. Author manuscript; available in PMC: 2019 Dec 3.
Published in final edited form as: Cancer Lett. 2018 Jun 15;432:216–226. doi: 10.1016/j.canlet.2018.06.011

Fig. 4. Srx contributed to urethane-induced BEAS2B cell transformation.

Fig. 4.

(A) Representative images and data of anchorage independent colony growth showed that urethane but not saline increased the number of colonies in soft agar. *Compared with control, p < 0.05 (n=6, one-way ANOVA). (B) Cells stably expressing a non-target ShRNA (ShNT) or ShRNA targeting Srx (ShSrx) were treated with saline or urethane. Western blot results showed efficient knockdown of Srx in urethane treated cells. (C) ShNT or ShSrx cells were cultured in the presence of urethane in soft agar and anchorage independent colonies were examined. *Compared with ShNT, p < 0.05 (n = 6, two way ANOVA). (D) Cells were treated with indicated concentration of urethane in the absence or presence of K27 (a specific inhibitor of Srx) for 24 h. Cells were harvested and the levels of hyperoxidized Prxs were examined. (E) Anchorage independent colony growth of cells treated with saline or urethane in the presence or absence of K27 at indicated concentration. *Compared with DMSO, p < 0.05 (n = 6, two way ANOVA).