KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-CTLA-4 (In vivo injection) | AbbVie | UC-10 |
| Anti-PD-1 (In vivo injection) | AbbVie | 17D2 |
| Anti-PD-1 (In vivo injection) | BioXCell | RMP1–14 |
| Anti-PD-1 DANA (In vivo injection) | AbbVie | 17D2 |
| Anti-PD-L1 (In vivo injection) | BioXCell | 10F.9G2 |
| CD3 | Biolegend | Cat#100232; RRID: AB_2562554 |
| CD4 | Biolegend | Cat#100447; RRID: AB_2564586 |
| CD8 | BD Bioscience | Cat#552877; RRID: AB_394506 |
| CD16/32 | TONBO | Cat#70–0161-U500; RRID: AB_2621487 |
| CD44 | Biolegend | Cat#103049; RRID: AB_2562600 |
| CD45 | Biolegend | Cat#103126; RRID: AB_493535 |
| CD45.1 | Biolegend | Cat#110737; RRID: AB_11204076 |
| CD45.2 | Biolegend | Cat#109847; RRID: AB_2616859 |
| CD62L | Biolegend | Cat#104433; RRID: AB_10900262 |
| Foxp3 | eBioscience | Cat#11–5773-82; RRID: AB_465243 |
| rIgG2a/k | eBioscience | Cat#11–4321; RRID: AB_470011 |
| PD-1 | Biolegend | Cat#135213; RRID: AB_10689633 |
| rIgG2a/k | Biolegend | Cat#400505; RRID: AB_2736919 |
| Tim-3 | Biolegend | Cat#134003; RRID: AB_1626181 |
| rIgG1/k | Biolegend | Cat#400407; RRID: AB_326513 |
| KLRG1 | Biolegend | Cat#138412; RRID: AB_10641560 |
| Hamster IgG | Biolegend | Cat#402012 |
| IFN-γ | BD Bioscience | Cat#554411; RRID: AB_395375 |
| Rat IgG1 | BD Bioscience | Cat#553924; RRID: AB_479706 |
| CD119 | BD Bioscience | Cat#740897; RRID: AB_2740545 |
| Rat IgG2a | BD Bioscience | Cat#563335 |
| Caspase-3 | BD Bioscience | Cat#51–68654X; RRID: AB_393697 |
| Spas-1 | NIH tetramer Core | Sequence: STHVNHLHCH-2D(b) |
| Spas-2 | NIH tetramer Core | Sequence: IIITFNDLH-2K(b) |
| CD45 (Cy-TOF) | Fluidigm | FLDM#3089003B; RRID: AB_2661851 |
| CD11c (Cy-TOF) | Fluidigm | FLDM#BL 337202 |
| CD235ab/CD61 (Cy-TOF) | Fluidigm | FLDM#BL 306602/BL 336402 |
| CD16 (Cy-TOF) | Fluidigm | FLDM#BL 302002 |
| CD45RA (Cy-TOF) | Fluidigm | FLDM#3143006B; RRID: AB_2651156 |
| CD4 (Cy-TOF) | Fluidigm | FLDM#3145001B; RRID: AB_2661789 |
| CD8a (Cy-TOF) | Fluidigm | FLDM#3146001B; RRID: AB_2687641 |
| BDCA-2 (Cy-TOF) | Fluidigm | FLDM#3147009B; RRID: AB_2714153 |
| CD14 (Cy-TOF) | Fluidigm | FLDM#3151009B |
| FoxP3 (Cy-TOF) | Fluidigm | FLDM#3162011A; RRID: AB_2687650 |
| CD25 (Cy-TOF) | Fluidigm | FLDM#3169003B; RRID: AB_2661806 |
| IFNg (Cy-TOF) | Fluidigm | FLDM#3165002B |
| CD127 (Cy-TOF) | Fluidigm | FLDM#3168017B |
| CD3 (Cy-TOF) | Fluidigm | FLDM#3170001B; RRID: AB_2661807 |
| HLA-DR (Cy-TOF) | Fluidigm | FLDM#3174001B; RRID: AB_2665397 |
| CD56 (Cy-TOF) | Fluidigm | FLDM#3176008B; RRID: AB_2661813 |
| CD27 (Cy-TOF) | Fluidigm | FLDM#3167006B |
| gdTCR (Cy-TOF) | Fluidigm | FLDM#BL331202 |
| TCRVd2 (Cy-TOF) | Fluidigm | FLDM#BL331402 |
| Human: MART-1 (IHC) | Novus Biotechne | Cat#NBP1–30151 |
| Human: CD8 (IHC) | Dako | Cat#M7103; RRID: AB_2075537 |
| Mouse: CD31 (IHC) | Invitrogen | Cat#MA1–40074 |
| Biological Samples | ||
| Blood samples from patients | Clinic | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DNase I | Sigma-Aldrich | Cat#D4263 |
| Xylenes | Fisher | Cat#X3P-1GAL |
| Citric acid monohydrate | Sigma-Aldrich | Cat#C1909–25G |
| TWEEN | Sigma-Aldrich | Cat#P7949–500ML |
| 30% Hydrogen Peroxide | Sigma-Aldrich | Cat#H1009–5ML |
| Gill’s Hematoxylin | Sigma-Aldrich | Cat#GHS216–500ML |
| Permount | Fisher | Cat#SP15–500 |
| DAB+ Substrate Chromagen System | Dako | Cat#K3468 |
| Streptavidin/Biotin Blocking Kit | Vector Laboratories | Cat#SP-2002 |
| Vectastain ABC Kit | Vector Laboratories | Cat#PK-4004 |
| Cas-Block | Life Technologies | Cat#008120 |
| 10X Tris-bufff Saline | Boston BioProducts | Cat#BM-300 |
| Antibody Diluent | Dako | Cat#S0809 |
| Recombinant Mouse PD-1 | R&D Systems | Cat#1021-PD |
| RNAlater | QIAGEN | Cat#76106 |
| Murine IL-2 | Peprotech | Cat#212–12 |
| Human IL-2 | Peprotech | Cat#200–02 |
| Recombinant Murine IFN-γ | Peprotech | Cat#315–05 |
| Recombinant Human IFN-γ | Peprotech | Cat#300–02 |
| 10 mM neutralized N-acetyl L-Cysteine | Sigma-Aldrich | Cat#A9165 |
| RBC Lysis Solution | Santa Cruz Biotechnology | Cat#SC-296258 |
| Collagenase IV | Sigma-Aldrich | Cat#C5138–5G |
| Critical Commercial Assays | ||
| Ambion micro RNA isolation kit | Ambion | Cat#AM1931 |
| Envision G2 Doublestain Kit | Dako | Cat#K5361 |
| RT2 PreAMP cDNA Synthesis Kit | QIAGEN | Cat#330451 |
| RT2 Profiler PCR Array Kit | QIAGEN | Cat#330522 |
| Splenocyte Isolation Kit | Miltenyi Biotec | Cat#130–095-130 |
| Human CD4 Isolation kit | STEMCELL Technologies | Cat#15062 |
| Human CD8 Isolation kit | STEMCELL Technologies | Cat#15063 |
| Mouse Cytokine 31-plex Discovery Assay | Eve Technologies | Cat#MD31 |
| Intracellular Staining Kit | eBioscience | Cat#00–5523-00 |
| Annexin V Apoptosis Detection Kit | BD Biosciences | Cat#556547 |
| Deposited Data | ||
| RNA sequence data | NCBI | GSE repository # 121694 |
| RT-PCR array data | NCBI | GSE repository # 95433 |
| Experimental Models: Cell Lines | ||
| Tramp-C2 | ATCC | Cat#CRL-2731; RRID: CVCL_3615 |
| MC-38 | Kerafast | Cat#ENH204; RRID: CVCL_B288 |
| MOC-1 | Gift from Dr. Uppaluri | MTA transfer from Dana-Farber |
| K562 | ATCC | Cat#CCL-243; RRID: CVCL_0004 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Ifngr KO | Jackson Laboratory | 003288 |
| Mouse: B6 Cd45.1 | Jackson Laboratory | 002014 |
| Mouse: C57BL/6j | Jackson Laboratory | 000664 |
| Oligonucleotides | ||
| Biotin-TSOLNA | Exiqon | N/A |
| Biotin-Oligo-dT30VN | Exiqon | N/A |
| Biotin-ISPCR oligo | Exiqon | N/A |
| read1_bc_1_GAGGTAG | Integrated DNA Technologies | N/A |
| read1_bc_2_GCTTAAC | Integrated DNA Technologies | N/A |
| read1_bc_3_GCAATTC | Integrated DNA Technologies | N/A |
| read1_bc_13_GATCCTA | Integrated DNA Technologies | N/A |
| read1_bc_14_TGGTCTC | Integrated DNA Technologies | N/A |
| read1_bc_15_TCCAGTC | Integrated DNA Technologies | N/A |
| read1_bc_25_ACACTTG | Integrated DNA Technologies | N/A |
| read1_bc_26_TACGGCA | Integrated DNA Technologies | N/A |
| read1_bc_27_TCTCGTG | Integrated DNA Technologies | N/A |
| read1_bc_37_GTCATCC | Integrated DNA Technologies | N/A |
| read1_bc_38_ACCGTAC | Integrated DNA Technologies | N/A |
| read1_bc_39_GTACTAC | Integrated DNA Technologies | N/A |
| read2_bc_1_GACGTAC | Integrated DNA Technologies | N/A |
| read2_bc_2_AACTGCA | Integrated DNA Technologies | N/A |
| read2_bc_3_CCACAGT | Integrated DNA Technologies | N/A |
| read2_bc_13_GGTCCTA | Integrated DNA Technologies | N/A |
| read2_bc_14_CCAACGA | Integrated DNA Technologies | N/A |
| read2_bc_15_AGGACAC | Integrated DNA Technologies | N/A |
| read2_bc_25_GTGTGTC | Integrated DNA Technologies | N/A |
| read2_bc_26_GCTACAA | Integrated DNA Technologies | N/A |
| read2_bc_27_CGTAGAC | Integrated DNA Technologies | N/A |
| read2_bc_37_CATGGAT | Integrated DNA Technologies | N/A |
| read2_bc_38_CTGCGAA | Integrated DNA Technologies | N/A |
| read2_bc_39_GAGACGA | Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad Software | N/A |
| FlowJo | TreeStar | https://www.flowjo.com/ |
| R | The R Foundation | https://www.r-project.org |
| Ingenuity Pathway Analysis | QIAGEN Bioinformatics | https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/ |
| StrataQuest | TissueGnostics | http://www.tissuegnostics.com/en/products/analysing-software/strataquest |
| Scaffold | Spitzer et al., 2017 | https://github.com/SpitzerLab/statisticalScaffold |
| CLC Genomics Workbench | QIAGEN Bioinformatics | https://www.qiagenbioinformatics.com/products/clc-genomics-workbench/ |
| Cytobank | Cytobank | https://www.cytobank.org |
| Other | ||
| Human T-Activator CD3/CD28 Dynabeads | Life Technologies | Cat#11131D |
| Recombinant human IFN-g protein | Peprotech | Cat#300–02 |
| Recombinant murine IFN-g protein | Peprotech | Cat#315–05 |